
Proceedings Paper
In vivo detection of microRNA within plants using plasmonic nanosensorsFormat | Member Price | Non-Member Price |
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Paper Abstract
Gene expression monitoring within whole plants is critical for many applications ranging from plant biology to biofuel development. Herein, we report a unique multimodal method for in vivo imaging and biosensing of nucleic acid biotargets, specifically microRNA, within whole plant leaves by integrating three complementary techniques: surface-enhanced Raman scattering (SERS), X-ray fluorescence (XRF), and plasmonics-enhanced two-photon luminescence (TPL). The method described utilizes plasmonic nanostar-based inverse molecular sentinel (iMS) nanoprobes, which not only provide large Raman signal enhancement upon target binding, but also allow for localization and quantification by XRF and plasmonics-enhanced TPL. This report lays the foundation for the use of plasmonic nanoprobes for in vivo functional imaging of nucleic acid biotargets in whole plants.
Paper Details
Date Published: 9 September 2019
PDF: 10 pages
Proc. SPIE 11082, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVII, 1108215 (9 September 2019); doi: 10.1117/12.2529818
Published in SPIE Proceedings Vol. 11082:
Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVII
Din Ping Tsai; Takuo Tanaka, Editor(s)
PDF: 10 pages
Proc. SPIE 11082, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVII, 1108215 (9 September 2019); doi: 10.1117/12.2529818
Show Author Affiliations
Bridget M. Crawford, Duke Univ. (United States)
Pietro Strobbia, Duke Univ. (United States)
Hsin-Neng Wang, Duke Univ. (United States)
Rodolfo Zentella, Duke Univ. (United States)
Maxim I. Boyanov, Institute of Chemical Engineering (Bulgaria)
Argonne National Lab. (United States)
Pietro Strobbia, Duke Univ. (United States)
Hsin-Neng Wang, Duke Univ. (United States)
Rodolfo Zentella, Duke Univ. (United States)
Maxim I. Boyanov, Institute of Chemical Engineering (Bulgaria)
Argonne National Lab. (United States)
Zhen-Ming Pei, Duke Univ. (United States)
Tai-Ping Sun, Duke Univ. (United States)
Kenneth M. Kemner, Argonne National Lab. (United States)
Tuan Vo-Dinh, Duke Univ. (United States)
Institute of Chemical Engineering (Bulgaria)
Tai-Ping Sun, Duke Univ. (United States)
Kenneth M. Kemner, Argonne National Lab. (United States)
Tuan Vo-Dinh, Duke Univ. (United States)
Institute of Chemical Engineering (Bulgaria)
Published in SPIE Proceedings Vol. 11082:
Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVII
Din Ping Tsai; Takuo Tanaka, Editor(s)
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