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Proceedings Paper

Optical trapping and manipulation with a low NA objective through 3D printed micro-mirrors (Conference Presentation)
Author(s): Gheorghe Cojoc; Andrea Bertoncini; Paul Müller; Jochen Guck; Carlo Liberale

Paper Abstract

In the last three decades, optical trapping techniques were heavily employed for contactless trapping and manipulation of biological samples. Dual-beam laser traps (DBLT) proved their convenience and became widely used as biophysical tool once a simplified experimental setup was proposed. This simplification was achieved by replacing the two objectives with optical fibers to deliver the two counter-propagating laser beams. However, fiber alignment can be inconvenient, time consuming and requires a lot of practice. Here, we present a novel way to overcome these issues by combining reconfigurable diffractive optical elements (DOE) and two photon lithography (2PL), using a single low NA objective. A single laser beam is divided into several beams by displaying a DOE on a spatial light modulator (SLM). This allows us to dynamically reconfigure the number of the beams, their shape, and relative 3D alignment. Furthermore, we use 3D printed micro-mirrors to direct the laser beams against each other and obtain a DBLT. The micro-mirrors were fabricated on top of a coverslip, by means of 2PL. Our preliminary results show the ability to trap dielectric and biological samples and their full 3D manipulation in a DBLT configuration. The ability to use DOEs to set the number of beams and their shape allow this technique to be coupled with novel forms of microscopy.

Paper Details

Date Published: 9 September 2019
Proc. SPIE 11083, Optical Trapping and Optical Micromanipulation XVI, 110830C (9 September 2019); doi: 10.1117/12.2529532
Show Author Affiliations
Gheorghe Cojoc, Biotechnologische Zentrum, TU Dresden (Germany)
Andrea Bertoncini, King Abdullah Univ. of Science and Technology (Saudi Arabia)
Paul Müller, Biotechnologisches Zentrum (Germany)
Jochen Guck, Biotechnologische Zentrum, TU Dresden (Germany)
Carlo Liberale, King Abdullah Univ. of Science and Technology (Saudi Arabia)

Published in SPIE Proceedings Vol. 11083:
Optical Trapping and Optical Micromanipulation XVI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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