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Motility assessment of green biflagellated microalgae in an optical trap using back focal plane interferometry
Author(s): Beatriz A. Juarez-Alvarez; Dann De la Torre; Veneranda G. Garces; Beatriz Cordero-Esquivel; Juan D. Sanchez-Lopez; Gabriel C. Spalding; Kevin A. O'Donnell
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Paper Abstract

Green biflagellated microalgae have proven to be of interest in biotechnology and biomedicine due to the production of lipids, carotenoids, and other components that have an environment dependent yield. In this work, we use back focal plane interferometry to obtain information about the behavior of microalgae held by an optical trap under different conditions. It has been observed that the elongated body of a microalga entering an optical trap will align along the beam axis and rotate counter-clockwise. The rotation is produced by the beating flagella, as we conclude from our observation of non-rotation of deflagellated or photodamaged cells. The dependence of rotation frequency on growth phase of the microalgae and on optical trapping power is investigated. To study these effects, each cell is held in the optical trap, and the laser light transmitted by the sample is collected with a microscope objective. Then, the back focal plane of the collection objective is imaged onto a quadrant photodiode. The voltage outputs of the photodiode are then recorded with a computer through the use of a custom Arduino circuit, and written to a text file for post-processing.

Paper Details

Date Published: 9 September 2019
PDF: 6 pages
Proc. SPIE 11083, Optical Trapping and Optical Micromanipulation XVI, 1108338 (9 September 2019); doi: 10.1117/12.2529506
Show Author Affiliations
Beatriz A. Juarez-Alvarez, Ctr. de Investigación Científica y de Educación Superior de Ensenada B.C. (Mexico)
Dann De la Torre, Univ. Autónoma de Baja California (Mexico)
Veneranda G. Garces, Pacifica Photonics (Mexico)
Beatriz Cordero-Esquivel, Ctr. de Investigación Científica y de Educación Superior de Ensenada B.C. (Mexico)
Juan D. Sanchez-Lopez, Univ. Autónoma de Baja California (Mexico)
Gabriel C. Spalding, Illinois Wesleyan Univ. (United States)
Kevin A. O'Donnell, Ctr. de Investigación Científica y de Educación Superior de Ensenada B.C. (Mexico)
Pacifica Photonics (Mexico)


Published in SPIE Proceedings Vol. 11083:
Optical Trapping and Optical Micromanipulation XVI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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