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In vivo x-ray imaging of the respiratory system using synchrotron sources and a compact light source
Author(s): Kaye Susannah Morgan; Regine Gradl; Martin Dierolf; Christoph Jud; Benedikt Günther; Freda Werdiger; Mark Gardner; Patricia Cmielewski; Alexandra McCarron; Nigel Farrow; Helena Haas; Melanie A. Kimm; Lin Yang; David Kutschke; Tobias Stoeger; Otmar Schmid; Klaus Achterhold; Franz Pfeiffer; David Parsons; Martin Donnelley
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Paper Abstract

Bright synchrotron x-ray sources enable imaging with short exposure times, and hence in a high-speed image sequence. These x-ray movies can capture not only sample structure, but also how the sample changes with time, how it functions. The use of a synchrotron x-ray source also provides high spatial coherence, which facilitates the capture of not only a conventional attenuation-based x-ray image, but also phase-contrast and dark-field signals. These signals are strongest from air/tissue interfaces, which means that they are particularly useful for examining the respiratory system. We have performed a range of x-ray imaging studies that look at lung function, airway surface function, inhaled and instilled treatment delivery, and treatment effect in live small animal models [Morgan, 2019]. These have utilized a range of optical set-ups and phase-contrast imaging methods in order to be sensitive to the relevant sample features, and be compatible with high-speed imaging. For example, we have used a grating interferometer to measure how the airsacs in the lung inflate during inhalation, via changes in the dark-field signal [Gradl, 2018], a single-exposure, single-grid set-up to capture changes in the liquid lining of the airways [Morgan, 2015] and propagation-based phase contrast to image clearance of inhaled debris [Donnelley, 2019]. Studies have also utilized a range of analysis methods to extract how the sample features change within a time-sequence of two-dimensional projections or three-dimensional volumes. While these imaging studies began in large-scale synchrotron facilities, we have recently performed these kinds of studies at an inverse-Compton-based compact synchrotron, the Munich Compact Light Source (MuCLS) [Gradl, 2018b]. 1. Morgan, Kaye, et al., “Methods for dynamic synchrotron X-ray imaging of live animals.”, under review 01/2019. 2. Gradl, R., et al. "Dynamic in vivo chest x-ray dark-field imaging in mice." IEEE Transactions on Medical Imaging (2018). 3. Morgan, Kaye S., et al. "In vivo X-ray imaging reveals improved airway surface hydration after a therapy designed for cystic fibrosis." American Journal of Respiratory and Critical Care Medicine 190.4 (2014): 469-472. 4. Donnelley, Martin, et al. "Live-pig-airway surface imaging and whole-pig CT at the Australian Synchrotron Imaging and Medical Beamline." Journal of Synchrotron Radiation 26.1 (2019). 5. Gradl, Regine, et al. "In vivo Dynamic Phase-Contrast X-ray Imaging using a Compact Light Source." Scientific Reports 8.1 (2018b): 6788.

Paper Details

Date Published: 19 September 2019
PDF: 12 pages
Proc. SPIE 11113, Developments in X-Ray Tomography XII, 111130G (19 September 2019); doi: 10.1117/12.2529276
Show Author Affiliations
Kaye Susannah Morgan, Monash Univ. (Australia)
Technische Univ. München (Germany)
Regine Gradl, Technische Univ. München (Germany)
Martin Dierolf, Technische Univ. München (Germany)
Christoph Jud, Technische Univ. München (Germany)
Benedikt Günther, Technische Univ. München (Germany)
Freda Werdiger, Monash Univ. (Australia)
Mark Gardner, Robinson Research Institute (Australia)
The Univ. of Adelaide (Australia)
Womens and Childrens Hospital (Australia)
Patricia Cmielewski, Robinson Research Institute (Australia)
The Univ. of Adelaide (Australia)
Women's and Children's Hospital (Australia)
Alexandra McCarron, Robinson Research Institute (Australia)
The Univ. of Adelaide (Australia)
Women's and Children's Hospital (Australia)
Nigel Farrow, Robinson Research Institute (Australia)
The Univ. of Adelaide (Australia)
Women's and Children's Hospital (Australia)
Helena Haas, Technische Univ. München (Germany)
Melanie A. Kimm, Technische Univ. München (Germany)
Lin Yang, Technische Univ. München (Germany)
David Kutschke, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Tobias Stoeger, Comprehensive Pneumology Ctr. (Germany)
Helmholtz Zentrum München GmbH (Germany)
Otmar Schmid, Comprehensive Pneumology Ctr. (Germany)
Helmholtz Zentrum München GmbH (Germany)
Klaus Achterhold, Technische Univ. München (Germany)
Franz Pfeiffer, Technische Univ. München (Germany)
David Parsons, Robinson Research Institute (Australia)
The Univ. of Adelaide (Australia)
Women's and Children's Hospital (Australia)
Martin Donnelley, Robinson Research Institute (Australia)
The Univ. of Adelaide (Australia)
Women's and Children's Hospital (Australia)


Published in SPIE Proceedings Vol. 11113:
Developments in X-Ray Tomography XII
Bert Müller; Ge Wang, Editor(s)

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