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Proceedings Paper

Video analysis of polymerized micro-cantilever deflected by the laser trap
Author(s): Zoltan Tomori; Veronika Kazikova; Jana Kubackova; Gregor Bánó
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Paper Abstract

Elastic micro-cantilever of 30-micrometer size is repeatedly deflected/released by optical tweezers trap, recorded by a high-speed camera (500 frames/sec) and subsequently processed off-line. This paper evaluates the position detection methods of the cantilever head, which is distorted by a diffraction pattern. We developed and tested four methods in our VideoAnalyser software - radial extremes, Hough transform, local corner tracking, and voting normal lines. The time dependence of the head position contains the information about the properties of both: cantilever material and the surrounding environment. Averaging of aligned graphs corresponding to individual cycles significantly improves the signal-to-noise ratio.

Paper Details

Date Published: 9 September 2019
PDF: 8 pages
Proc. SPIE 11083, Optical Trapping and Optical Micromanipulation XVI, 1108336 (9 September 2019); doi: 10.1117/12.2529129
Show Author Affiliations
Zoltan Tomori, Institute of Experimental Physics SAS (Slovakia)
Veronika Kazikova, Institute of Experimental Physics SAS (Slovakia)
Jana Kubackova, Institute of Experimental Physics SAS (Slovakia)
Gregor Bánó, Pavol Jozef Šafárik Univ. in Košice (Slovakia)

Published in SPIE Proceedings Vol. 11083:
Optical Trapping and Optical Micromanipulation XVI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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