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3D morphology of melanoma cells using digital holographic interferometry
Author(s): Natalith Palacios-Ortega; Fernando Mendoza Santoyo; J. Mauricio Flores Moreno; María del Socorro Hernández-Montes; Manuel H. De la Torre Ibarra; Germán Plascencia
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Paper Abstract

Depending on its stage skin cancer can be diagnosed through the so called ABCDE rule, which is mainly qualitative and noninvasive; besides this, there exists a technique that uses a dermatoscope to analyze the damaged area considered as noninvasive. On the other hand, the invasive methods mainly use sample-biopsies extracted from the affected area which are usually analyzed with a microscope or other imaging techniques. Digital holographic interferometry (DHI) has shown to be a powerful technique to obtain 3D information of a sample, and in these times, as many other optical metrology techniques, it has been widely applied to study biologic samples. In this research work, we present some initial 3D morphology measurements of melanoma cells made with a DHI setup implemented in a microscope type arrangement so a magnified image of the sample is studied. The morphology data depict the actual 3D contour map of the cell nucleus and represents a further advancement in the application of DHI to accurately study and measure the structure of cells.

Paper Details

Date Published: 3 September 2019
PDF: 6 pages
Proc. SPIE 11102, Applied Optical Metrology III, 111020H (3 September 2019); doi: 10.1117/12.2529000
Show Author Affiliations
Natalith Palacios-Ortega, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
Fernando Mendoza Santoyo, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
J. Mauricio Flores Moreno, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
María del Socorro Hernández-Montes, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
Manuel H. De la Torre Ibarra, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
Germán Plascencia, The Univ. of Texas at San Antonio (United States)


Published in SPIE Proceedings Vol. 11102:
Applied Optical Metrology III
Erik Novak; James D. Trolinger, Editor(s)

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