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Proceedings Paper

Stretching and characterization of human red blood cells with tunable “tug-of-war” optical tweezers (Conference Presentation)
Author(s): Yi Liang; Yinxiao Xiang; Josh Lamstein; Anna Bezryadina; Zhigang Chen

Paper Abstract

Understanding the deformability and associated biomechanical properties of red blood cells (RBCs) is crucial for many pathological analysis and diagnosis of human diseases. In such endeavors, optical tweezers have played an active role over the past decades. Here, we study the RBC deformability by employing a novel “tug of war” (TOW) optical tweezers consist of a pair of elongated diverging accelerating beams that can stably trap and stretch a single RBC under different osmotic conditions without any tethering or mechanical movement. With a viscous drag method, we compare directly the trapping force at different states of RBCs, and find that even one arm of the TOW tweezers can apply a force of over 18pN with only 100mW laser power, more than 2 times stronger than that from the Gaussian trap at the same condition. Without the need of two independent controls as in a conventional dual trap, the spacing between the two TOW traps can be increased conveniently from 0 to over 9m, resulting in nearly 15% of cell deformation. We obtain the shear modulus of the RBCs in different osmotic conditions, with the largest value of 3.36±0.95pN/μm in the hypertonic case, and compare with those previously reported results. Our work may bring about a new photonic tool for the study of biomechanical properties of living cells, promising for applications such as distinguishing healthy and diseased cells.

Paper Details

Date Published: 9 September 2019
Proc. SPIE 11083, Optical Trapping and Optical Micromanipulation XVI, 110830O (9 September 2019); doi: 10.1117/12.2528534
Show Author Affiliations
Yi Liang, San Francisco State Univ. (United States)
Guangxi Univ. (China)
Nankai Univ. (China)
Yinxiao Xiang, San Francisco State Univ. (United States)
Nankai Univ. (China)
Josh Lamstein, San Francisco State Univ. (United States)
Anna Bezryadina, San Francisco State Univ. (United States)
California State Univ., Northridge (United States)
Zhigang Chen, San Francisco State Univ. (United States)
Nankai Univ. (China)

Published in SPIE Proceedings Vol. 11083:
Optical Trapping and Optical Micromanipulation XVI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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