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Proceedings Paper

High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)
Author(s): Stylianos E. Psycharakis; Evangelos Liapis; Athanasios Zacharopoulos; Mariam-Eleni Oraiopoulou; Chrysoula Aivalioti; Vangelis Sakkalis; Joseph Papamatheakis; Jorge Ripoll; Giannis Zacharakis
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Paper Abstract

Breast cancer and Glioblastoma brain cancer are aggressive malignancies with poor prognosis. In this study primary Glioblastoma and secondary breast cancer spheroids are formed and treated with the well-known Temozolomide and Doxorubicin chemotherapeutics, respectively. A custom multi-angle Light Sheet Fluorescence Microscope is employed for high resolution imaging of both cancer cell spheroids. Such a technique is successful in realizing pre-clinical drug screening, while enables the discrimination among physiologic tumor parameters. LSFM technique, parameters and method followed are also presented.

Paper Details

Date Published: 22 July 2019
PDF: 6 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760O (22 July 2019); doi: 10.1117/12.2527214
Show Author Affiliations
Stylianos E. Psycharakis, Foundation for Research and Technology-Hellas (Greece)
Univ. of Crete (Greece)
Evangelos Liapis, Foundation for Research and Technology-Hellas (Germany)
Helmholtz Zentrum München (Germany)
Athanasios Zacharopoulos, Foundation for Research and Technology-Hellas (Greece)
Mariam-Eleni Oraiopoulou, Foundation for Research and Technology-Hellas (Greece)
Chrysoula Aivalioti, Univ. of Crete (Greece)
Vangelis Sakkalis, Foundation for Research and Technology-Hellas (Greece)
Joseph Papamatheakis, Foundation for Research and Technology-Hellas (Greece)
Jorge Ripoll, Univ. Carlos III de Madrid (Spain)
Giannis Zacharakis, Foundation for Research and Technology-Hellas (Greece)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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