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Proceedings Paper

Fast P-THG microscopy for the characterization of biomaterials
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Paper Abstract

The application range of P-THG microscopy has been so far restricted to studies on molecular order and anisotropy of static specimen removed from their biological environment. Slow polarization commutation limits the investigation of highly dynamic systems because of motion artifacts. Here we have developed a new fast-P-THG microscope enabling efficient in vivo studies in dynamic biological samples. Our P-THG scheme benefits from a built-in EOM that switch polarization states at kilohertz between image lines to provide artefact-free P-THG images with micrometric resolution. Furthermore, we have developed a fast Fourier analysis enabling rapid P-THG processing to quantify lipid order and angular maps. We demonstrated that fast-P-THG is suitable in two major applications. Using first a linear polarization configuration, fast P-THG imaging revealed molecular order changes in MLVs undergoing phase-transition upon heating despite sample distortions. Anisotropy properties of small endogenous microparticles swimming in the otolith cavity embryos were also reported in early zebrafish embryos. A second configuration with linear-circular polarization commutation enabled efficient detection of birefringent media such as anisotropic vesicles in C-elegans gut cells.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 1107613 (22 July 2019); doi: 10.1117/12.2527209
Show Author Affiliations
Joséphine Morizet, Ecole Polytechnique, Lab for Optics and Biosciences, CNRS, INSERM (France)
Guillaume Ducourthial, Ecole Polytechnique, Lab for Optics and Biosciences, CNRS, INSERM (France)
Willy Supatto, Ecole Polytechnique, Lab for Optics and Biosciences, CNRS, INSERM (France)
Arthur Boutillon, Ecole Polytechnique, Lab for Optics and Biosciences, CNRS, INSERM (France)
Renaud Legouis, Institute for Integrative Biology of the Cell, CEA, CNRS, Univ. Paris-Sud, Univ. Paris-Saclay (France)
Marie-Claire Schanne-Klein, Ecole Polytechnique, Lab for Optics and Biosciences, CNRS, INSERM (France)
Chiara Stringari, Ecole Polytechnique, Lab for Optics and Biosciences, CNRS, INSERM (France)
Emmanuel Beaurepaire, Ecole Polytechnique, Lab for Optics and Biosciences, CNRS, INSERM (France)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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