For performance testing of high-resolution label-free optical coherence tomography (OCT) systems, durable test standards with optical properties similar to native biological tissues are highly desirable. Therefore, we explored the establishment of ex-vivo 3D samples from dissected mouse retina. To achieve high stability, dissected tissue parts were embedded in EPON resin. Results from comparative investigations with OCT on murine retina in agarose demonstrate that by utilization of appropriate EPON embedding protocols durable samples with adequate refractive index properties can be created in which even tiny structures near the OCT resolution limits can be detected.

Date Published: 19 July 2019
PDF: 3 pages
Proc. SPIE 11078, Optical Coherence Imaging Techniques and Imaging in Scattering Media III, 110781V (19 July 2019); doi: 10.1117/12.2527163

Published in SPIE Proceedings Vol. 11078:
Optical Coherence Imaging Techniques and Imaging in Scattering Media III
Maciej Wojtkowski; Stephen A. Boppart; Wang-Yuhl Oh, Editor(s)