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Proceedings Paper

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method
Author(s): Irene Costantini; Giacomo Mazzamuto; Annunziatina Laurino; Erica Lazzeri; Leonardo Sacconi; Mattia Neri; Andrea Simonetto; Matteo Roffilli; Ludovico Silvestri; Francesco S. Pavone
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Paper Abstract

The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760B (22 July 2019);
Show Author Affiliations
Irene Costantini, European Lab. for Non-Linear Spectroscopy (Italy)
National Institute of Optics, National Research Council (Italy)
Giacomo Mazzamuto, European Lab. for Non-Linear Spectroscopy (Italy)
National Institute of Optics, National Research Council (Italy)
Annunziatina Laurino, European Lab. for Non-Linear Spectroscopy (Italy)
Univ. of Florence (Italy)
Erica Lazzeri, European Lab. for Non-Linear Spectroscopy (Italy)
Univ. of Florence (Italy)
Leonardo Sacconi, European Lab. for Non-Linear Spectroscopy (Italy)
National Institute of Optics, National Research Council (Italy)
Mattia Neri, Bioretics srl (Italy)
Andrea Simonetto, Bioretics srl (Italy)
Matteo Roffilli, Bioretics srl (Italy)
Ludovico Silvestri, European Lab. for Non-Linear Spectroscopy (Italy)
National Institute of Optics, National Research Council (Italy)
Francesco S. Pavone, European Lab. for Non-Linear Spectroscopy (Italy)
National Institute of Optics, National Research Council (Italy)
Univ. of Florence (Italy)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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