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Proceedings Paper

Increase and homogenization of the endogenous production of protoporphyrin IX by photobiomodulation
Author(s): Jaroslava Joniová; Emmanuel Gerelli; Georges Wagnières
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Paper Abstract

Photodynamic therapy (PDT) and/or photodetection (PDD) based on protoporphyrin IX (PpIX) are used in many medical fields. However, the endogenous production of PpIX in the tissue after administration of its precursor, 5-aminolevulinic acid (ALA), is frequently insufficient and, in particular, inhomogeneous, leading to unsatisfactory treatment outcomes or false negatives. Photobiomodulation (PBM), which is based on the application of a sub-thermal dose of red or near infrared light (typically in the range of 600 – 900 nm), is known to modulate, among others, the cell metabolism, as demonstrated by an increased production of adenosine triphosphate and changes of the mitochondrial potential. Yet, the precise mechanism of PBM and, furthermore, the optimal irradiation conditions are to be determined. In the work reported here, we have studied the dependence of the endogenous PpIX production by U-87 glioma cells on various PBM irradiation protocols (wavelengths, irradiations, light doses). We have shown that PBM can increase, and even homogenize the endogenous production of PpIX in these cells. Therefore, combining PBM with PDT or PDD could lead to more potent and satisfying cancer treatments or detections outcomes, in particular in neurosurgery, dermatology, and urology.

Paper Details

Date Published: 22 July 2019
PDF: 4 pages
Proc. SPIE 11079, Medical Laser Applications and Laser-Tissue Interactions IX, 110790Q (22 July 2019); doi: 10.1117/12.2527082
Show Author Affiliations
Jaroslava Joniová, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Emmanuel Gerelli, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Georges Wagnières, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 11079:
Medical Laser Applications and Laser-Tissue Interactions IX
Lothar D. Lilge; Carsten M. Philipp, Editor(s)

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