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Proceedings Paper

Myeloarchitecture of the murine neocortex visualized by intrinsic third-harmonic generation
Author(s): Michael Redlich; Hyungsik Lim
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Paper Abstract

Myelination in the brain has been typically associated with the white matter. Recently, however, cortical myelination has gained much interest not only as a possible lesion of multiple sclerosis (MS) but also for its significance in the plasticity of the central nervous system (CNS). It is urgent to understand the functional role of cortical myelination and its involvement in the MS pathogenesis. Here we demonstrate a novel nonlinear optical contrast, namely third-harmonic generation (THG), as a basis of label-free imaging of cortical myelin in living animals. Using the fixed brain slices of fluorescently labeled transgenic mice, we examined the distribution of myelin in the normal brain. We measured the spatial organization of cortical myelin. Finally, the capability of THG microscopy was tested for visualizing cortical myelin in living, unlabeled animals.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760A (22 July 2019); doi: 10.1117/12.2527064
Show Author Affiliations
Michael Redlich, Hunter College (United States)
The Graduate Ctr. of the City Univ. of New York (United States)
Hyungsik Lim, Hunter College (United States)
The Graduate Ctr. of the City Univ. of New York (United States)

Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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