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Proceedings Paper

Probing living cells by terahertz attenuated total reflection: application to permeabilization dynamics
Author(s): G. Gallot; X. Zheng; M. Grognot; A. Azan; T. Garcia-Sanchez; L. Descamps; L. M. Mir
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Paper Abstract

Using attenuated total reflection (ATR) in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live epithelial cells by saponin, and after electropermeabilization. The origin of the contrast observed between cells and culture medium is addressed by both experimental and theoretical approaches, and gives access to permeabilization dynamics of live cells in real time. We show that terahertz modalities are more sensitive than fluorescence microscopy which is the reference optical technique for electropermeabilization. We propose analytical models for the influx and efflux of non-permeant molecules through permeabilized cell membranes.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11075, Novel Biophotonics Techniques and Applications V, 110750E (22 July 2019); doi: 10.1117/12.2526722
Show Author Affiliations
G. Gallot, Lab. d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM (France)
X. Zheng, Lab. d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM (France)
M. Grognot, Lab. d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM (France)
A. Azan, CNRS, Gustave Roussy, Univ. Paris-Sud, Univ. Paris-Saclay (France)
T. Garcia-Sanchez, CNRS, Gustave Roussy, Univ. Paris-Sud, Univ. Paris-Saclay (France)
L. Descamps, CNRS, Gustave Roussy, Univ. Paris-Sud, Univ. Paris-Saclay (France)
L. M. Mir, CNRS, Gustave Roussy, Univ. Paris-Sud, Univ. Paris-Saclay (France)


Published in SPIE Proceedings Vol. 11075:
Novel Biophotonics Techniques and Applications V
Arjen Amelink; Seemantini K. Nadkarni, Editor(s)

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