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Proceedings Paper

Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes
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Paper Abstract

Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limited-angle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a nonhomogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.

Paper Details

Date Published: 21 June 2019
PDF: 10 pages
Proc. SPIE 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV, 1106016 (21 June 2019); doi: 10.1117/12.2525840
Show Author Affiliations
José A. Rodrigo, Univ. Complutense de Madrid (Spain)
Juan M. Soto, Univ. Complutense de Madrid (Spain)
Tatiana Alieva, Univ. Complutense de Madrid (Spain)

Published in SPIE Proceedings Vol. 11060:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV
Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; Christoph K. Hitzenberger, Editor(s)

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