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Proceedings Paper

Automatized fine-focusing in digital microscopy
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Paper Abstract

This paper proposes a procedure for automatized fine-focusing of particles’ image in digital microscopy. First, analytical formulas for a calculation of axial defocus are derived using the complex amplitude propagation. Afterwards, the formulas are used for a design of an iterative algorithm for the fine-focusing. Uncertainty analysis and error simulations are then presented. In the final part of the paper, the method is verified by a simple experimental setup. The proposed strategy can find utilisation in many practical applications of automatized processes, not only in digital microscopy.

Paper Details

Date Published: 21 June 2019
PDF: 12 pages
Proc. SPIE 11062, Digital Optical Technologies 2019, 110621I (21 June 2019); doi: 10.1117/12.2525421
Show Author Affiliations
Petr Pokorný, Czech Technical Univ. in Prague (Czech Republic)
Filip Šmejkal, Czech Technical Univ. in Prague (Czech Republic)
Pavel Novák, Czech Technical Univ. in Prague (Czech Republic)
Jirí Novák, Czech Technical Univ. in Prague (Czech Republic)
Antonín Mikš, Czech Technical Univ. in Prague (Czech Republic)


Published in SPIE Proceedings Vol. 11062:
Digital Optical Technologies 2019
Bernard C. Kress; Peter Schelkens, Editor(s)

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