Understanding free-electron mediated effects of tightly focused femtosecond pulse series is essential for minimizing photodamage in nonlinear microscopy and opens new avenues for nanosurgery and intentional modifications of biomolecules. We tracked different stages of the photomodification kinetics (hyperfluorescence, plasma luminescence, bubble formation) by time-lapse 2-photon microscopy, fluorescence lifetime measurements, and bubble interferometry with nanometer resolution. Monitoring of bubble growth during pulse series enabled us to quantify chemical reaction rates leading to gas formation via molecular disintegration. Novel ways of data evaluation were used to create a comprehensive picture of the photomodification kinetics in the (irradiance/irradiation dose) parameter space.

Date Published: 8 March 2019
PDF
Proc. SPIE 10876, Optical Interactions with Tissue and Cells XXX, 1087612 (8 March 2019); doi: 10.1117/12.2525153

Published in SPIE Proceedings Vol. 10876:
Optical Interactions with Tissue and Cells XXX
Hope Thomas Beier; Bennett L. Ibey, Editor(s)