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Proceedings Paper

Cell trauma detection using infra-red live cell imaging
Author(s): Ben O. L. Mellors; Hamid Dehghani; Christopher R. Howle; Abigail M. Spear
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Paper Abstract

Infra-red (IR) spectroscopic imaging of live cells is greatly affected by the presence of water, which is a strong absorber of IR radiation. In order to overcome this, a variety of methods have been developed using complex microfluidic devices to reduce the liquid sample path length. However, these devices are often custom made needing both specialised equipment and detailed fabrication steps. Here we show the novel utilisation of a liquid-air interface configuration and a negative contrast imaging device (NCI) reflectance imaging system for the collection of spectral data from live cells within an in vitro environment. Spectral differences were observed between two different cell densities, both in the presence and absence of cell culture media. Additionally, differences were observed between control and test cultures exposed to dimethyl sulfoxide (DMSO) to induce cell apoptosis. The NCI system acquired data in the 2.5 – 3.5 μm spectral region, at a spectral sampling interval of 10 nm. This method will allow further investigation of spectral biomarkers within cell cultures to augment understanding of specific cell contributions to wound healing in vivo.

Paper Details

Date Published: 19 July 2019
PDF: 3 pages
Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110730M (19 July 2019); doi: 10.1117/12.2525012
Show Author Affiliations
Ben O. L. Mellors, The Univ. of Birmingham (United Kingdom)
Hamid Dehghani, The Univ. of Birmingham (United Kingdom)
Christopher R. Howle, Defence Science and Technology Lab. (United Kingdom)
Abigail M. Spear, Defence Science and Technology Lab. (United Kingdom)

Published in SPIE Proceedings Vol. 11073:
Clinical and Preclinical Optical Diagnostics II
J. Quincy Brown; Ton G. van Leeuwen, Editor(s)

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