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Proceedings Paper

Light sheet microscopy of blood vessels in mouse brain in vivo
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Paper Abstract

A method for intravital light sheet microscopy of blood vessels of mouse brain cortex is proposed. The use of tilted microscope lens mount and long working distance of microscope lens ensure high contrast images in both scattered light and fluorescence modes without immersion. Fluctuations of laser speckles related with the blood flow has been observed in scattered mode. In fluorescence mode distribution of Evans Blue dye over blood vessel cross-section was visualized.

Paper Details

Date Published: 3 June 2019
PDF: 6 pages
Proc. SPIE 11067, Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions, 1106711 (3 June 2019); doi: 10.1117/12.2523883
Show Author Affiliations
Ivan V. Fedosov, Saratov State Univ. (Russian Federation)
Oxana V. Semyachkina-Glushkovskaya, Saratov State Univ. (Russian Federation)
Valery V. Tuchin, Saratov State Univ. (Russian Federation)
Tomsk State Univ. (Russian Federation)
Institute of Precision Mechanics and Control (Russian Federation)


Published in SPIE Proceedings Vol. 11067:
Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions
Dmitry Engelevich Postnov, Editor(s)

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