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Proceedings Paper

Spectrophotometric vs. colorimetric analysis of mycobacterium tuberculosis population growth curves in resazurin assay
Author(s): Eugene B. Postnikov; Anastasia I. Lavrova; Andrey A. Khalin; Marine Z. Dogonadze; Olga A. Manicheva
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Paper Abstract

The rezazurin assay for screening of bacterial growth remains the most popular method for express diagnostic tests. It is based on the change of colour and uorescence properties of the respective dye due to bacterial breathing but its conventional quantitative practical implementation requires a relative expensive and cumbersome spectrophotometric equipment. As an alternative approach, we propose to shift a focus from hardware to software. Preliminary results announced in this work show that the processing RGB colour channels for simple photographic images of resazurin assay plates with mycobacterium tuberculosis provides an opportunity to reconstruct the target growth curve with an accuracy comparable with results obtained using the conventional photometric uorescence curves.

Paper Details

Date Published: 3 June 2019
PDF: 7 pages
Proc. SPIE 11067, Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions, 110670L (3 June 2019); doi: 10.1117/12.2523300
Show Author Affiliations
Eugene B. Postnikov, Kursk State Univ. (Russian Federation)
Anastasia I. Lavrova, Saint Petersburg State Univ. (Russian Federation)
Saint Petersburg State Research Institute of Phthisiopulmonology (Russian Federation)
Andrey A. Khalin, Kursk State Univ. (Russian Federation)
Marine Z. Dogonadze, Saint Petersburg State Research Institute of Phthisiopulmonology (Russian Federation)
Olga A. Manicheva, Saint Petersburg State Research Institute of Phthisiopulmonology (Russian Federation)


Published in SPIE Proceedings Vol. 11067:
Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions
Dmitry Engelevich Postnov, Editor(s)

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