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Proceedings Paper

Improvement of optical setup for microcirculation imaging and flow analysis in septic shock rats
Author(s): Mami Kawasaki; Ryohei Hashimoto; Kazuya Nakano; Takashi Ohnishi; Masashi Sekine; Eizo Watanabe; Shigeto Oda; Hideaki Haneishi
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Paper Abstract

Microcirculation plays an important role in maintaining our lives. Observing the microcirculation has been considered important in understanding the disease mechanisms and diagnosing diseases. Sidestream dark-field (SDF) imaging is one of the methods to observe the microcirculation. However, the SDF imaging has several problems for instance artifacts caused by pressure and heat. Measurement points is under pressure because SDF imaging requires direct contact with measurement points, which may affect hemodynamics. Therefore, we construct a non-contact setup. Furthermore, at the early stage of sepsis, it is known that the microcirculation is impaired. To investigate the relationship between the flow of red blood cells (RBCs) and septic shock, we conducted an experiment using the setup to observe septic model rats and sham rats. Moreover, we calculated the blood velocity to estimate the flow of RBCs by using acquired motion pictures. We confirmed that the sham rats showed slight change in lactate value during the observation and improved the blood velocity compared with just after abdominal closure. However, lactate value of the septic model rats increased and the blood velocity of septic model rats decreased. This finding suggests that microcirculatory alteration may be a sign of sepsis and septic shock progression.

Paper Details

Date Published: 27 March 2019
PDF: 6 pages
Proc. SPIE 11050, International Forum on Medical Imaging in Asia 2019, 1105009 (27 March 2019); doi: 10.1117/12.2521514
Show Author Affiliations
Mami Kawasaki, Chiba Univ. (Japan)
Ryohei Hashimoto, Chiba Univ. (Japan)
Kazuya Nakano, Chiba Univ. (Japan)
Takashi Ohnishi, Chiba Univ. (Japan)
Masashi Sekine, Chiba Univ. (Japan)
Eizo Watanabe, Chiba Univ. (Japan)
Shigeto Oda, Chiba Univ. (Japan)
Hideaki Haneishi, Chiba Univ. (Japan)

Published in SPIE Proceedings Vol. 11050:
International Forum on Medical Imaging in Asia 2019
Feng Lin; Hiroshi Fujita; Jong Hyo Kim, Editor(s)

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