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Proceedings Paper

3D visualization in multifocus fluorescence microscopy
Author(s): Julia R. Alonso; Alejandro Silva; Miguel Arocena
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Paper Abstract

Limited depth-of-field can be overcome through computational optical imaging. In this work, a custom built fluorescence microscope with a focus electrically tunable lens is used for the acquisition of a multifocus image sequence (z-stack) of a 3D fluorescent sample. Image registration between the acquired images is often needed as a preprocessing step before the reconstruction of images with new characteristics. Then a multifocus image fusion algorithm is implemented to accomplish all-in-focus image reconstruction from the registered z-stack. Also computational perspective shifts that allow to the reconstruction of stereoscopic pairs of the sample and its three-dimensional visualization are implemented.

Paper Details

Date Published: 14 May 2019
PDF: 8 pages
Proc. SPIE 10997, Three-Dimensional Imaging, Visualization, and Display 2019, 109970Q (14 May 2019); doi: 10.1117/12.2520067
Show Author Affiliations
Julia R. Alonso, Univ. de la República Uruguay (Uruguay)
Alejandro Silva, Univ. de la República Uruguay (Uruguay)
Miguel Arocena, Univ. de la República Uruguay (Uruguay)
Instituto de Investigaciones Biológicas Clemente Estable (Uruguay)

Published in SPIE Proceedings Vol. 10997:
Three-Dimensional Imaging, Visualization, and Display 2019
Bahram Javidi; Jung-Young Son; Osamu Matoba, Editor(s)

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