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Proceedings Paper

Gas-phase biosensors (bio-sniffer and sniff-cam) for volatile chemicals
Author(s): Kohji Mitsubayashi
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Paper Abstract

Gas-phase biosensors (Bio-sniffers, Sniff-cam) have been investigated not only for human volatiles (acetone, methyl mercaptan, trimethylamine, ethanol, isopropanol, etc.) but also for residential harmful VOCs (formaldehyde, toluene, nicotine) causing sick-house syndrome, etc. The biofluorometric bio-sniffers constructed with UV-LED and PMT shows good sensitivity and selectivity for continuous monitoring of target VOCs (formaldehyde, ethanol, acetaldehyde, acetone, isopropanol, etc.). The sniff-cam with enzyme immobilized mesh demonstrates a spatiotemporal gas-imaging for human volatiles (i.e. ethanol, acetaldehyde, etc. after drinking). As novel non-invasive biosensing approaches, the gas-phase biosensors for human and environmental VOCs will be introduced in this contribution. The bio-sniffer for acetone vapor was developed using S-ADH (secondary alcohol dehydrogenase) reverse reaction by detecting NADH fluorometric system. The S-ADH was possible to continuous measure gaseous acetone from less than 1 ppb to 20ppm with a good selectivity based on the enzyme specificity. The device allows to use the evaluation of the acetone concentration in exhaled air from healthy subjects and diabetes patients (type I and II). The novel biofluorometric sniff-cam for ethanol was also fabricated with ADH (alcohol dehydrogenase) immobilized mesh and the NADH visualization unit (UV-LED sheet array and high sensitive CCD), thus imaging human ethanol vapor not only exhaled air but also skin gas after drinking. The sniffer-device would be useful for conventional detecting the volatile biomarkers.

Paper Details

Date Published: 10 May 2019
PDF: 6 pages
Proc. SPIE 11007, Advanced Environmental, Chemical, and Biological Sensing Technologies XV, 110070C (10 May 2019); doi: 10.1117/12.2519182
Show Author Affiliations
Kohji Mitsubayashi, Tokyo Medical and Dental Univ. (Japan)

Published in SPIE Proceedings Vol. 11007:
Advanced Environmental, Chemical, and Biological Sensing Technologies XV
Tuan Vo-Dinh, Editor(s)

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