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Proceedings Paper

Additive manufacturing of catalytically active living material hydrogels
Author(s): Trevor G. Johnston; Christopher R. Fellin; Alberto Carignano; Alshakim Nelson
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Paper Abstract

Herein, we describe a yeast-laden hydrogel ink that can be printed using a direct-write 3D printer and used for the production of a peptide. A poly(alkyl glycidyl ether)-based triblock copolymer was synthesized and formulated as a hydrogel that was characterized via rheometry to evaluate the printability of the hydrogel ink. An engineered yeast strain with an upregulated α-factor production pathway was incorporated into the hydrogel ink and 3D printed. The immobilized yeast cells exhibited adequate viability of 87.5% within the hydrogel. The production of the up-regulated α- factor was detected using a detecting yeast strain and quantified at 268 nM (s = 34.6 nM) over 72 h. The reusability of these systems was demonstrated by immersion of the yeast-laden hydrogel lattice in fresh SC media and confirmed by the detection of similar amounts of up-regulated α-factor 259 nM (s = 45.1 nM). These yeast-laden materials represent an attractive opportunity for whole-cell catalysis of other high-value products in a sustainable and continuous manner.

Paper Details

Date Published: 13 May 2019
PDF: 8 pages
Proc. SPIE 10982, Micro- and Nanotechnology Sensors, Systems, and Applications XI, 109820B (13 May 2019); doi: 10.1117/12.2518653
Show Author Affiliations
Trevor G. Johnston, Univ. of Washington (United States)
Christopher R. Fellin, Univ. of Washington (United States)
Alberto Carignano, Univ. of Washington (United States)
Alshakim Nelson, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 10982:
Micro- and Nanotechnology Sensors, Systems, and Applications XI
Thomas George; M. Saif Islam, Editor(s)

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