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Proceedings Paper

A bimodal bioluminescent Ca2+ indicator toward spatiotemporally scalable imaging
Author(s): Israt Farhana; Kazushi Suzuki; Tomoki Matsuda; Takeharu Nagai
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Paper Abstract

For decades, genetically encoded Ca2+ indicators (GECIs), have been under development for the purpose of visualizing intracellular Ca2+ dynamics. Fluorescent GECIs are limited for the purpose of deep tissue long-term whole-body imaging due to their requirement for external illumination. Bioluminescent GECIs overcome these restraints but are somewhat compromised in terms of spatiotemporal resolution compared to fluorescent probes. To address this, we developed a bimodal Ca2+ indicator by combining a single fluorescent protein based Ca2+ indicator and a split luciferase. The novel design of this bimodal indicator enables Ca2+ imaging in the same specimen in both fluorescent and bioluminescent mode. The ability to switch between fluorescent and bioluminescent modes with a single indicator should benefit applications where micro and macro scale observation of cells or tissues is desirable. Use of such a probe enables trans-scale imaging, where macro scale imaging of a group of cells or tissue can be combined with fluorescent imaging of single cells.

Paper Details

Date Published: 22 February 2019
PDF: 6 pages
Proc. SPIE 10866, Optogenetics and Optical Manipulation 2019, 1086603 (22 February 2019); doi: 10.1117/12.2518178
Show Author Affiliations
Israt Farhana, Osaka Univ. (Japan)
Kazushi Suzuki, Osaka Univ. (Japan)
Tomoki Matsuda, Osaka Univ. (Japan)
Takeharu Nagai, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 10866:
Optogenetics and Optical Manipulation 2019
Samarendra K. Mohanty; E. Duco Jansen, Editor(s)

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