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Proceedings Paper

3D phase imaging for thick biological samples (Conference Presentation)
Author(s): Regina Eckert; Michael Chen; Li-Hao Yeh; Laura Waller

Paper Abstract

Phase imaging provides quantitative structural data about biological samples as an alternative or complementary contrast method to the functional information given by fluorescence imaging. In certain cases, fluorescence imaging is undesirable because it may harm the development of living cells or add time and complexity to imaging pipelines. However, current 3D phase reconstruction methods, such as optical diffraction tomography [1], are often limited to a single-scattering approximation. This limits the amount of scattering that such 3D reconstruction algorithms can successfully handle, and therefore effectively limits the sample thickness that can be successfully reconstructed. More recent methods such as 3D Fourier ptychographic microscopy (FPM) have used intensity-only images combined with multiple-scattering models in order to reconstruct 3D volumes [2]. In practice, however, continuous biological samples on the order of 100 um thick are not well-reconstructed by 3D FPM, due to a lack of diverse information across the volume which creates an ill-posed inverse problem. To mitigate this, we introduce simultaneous detection coding in the form of pupil control to the 3D FPM capture scheme. Simple pupil coding schemes enabled us to capture diverse information across our volume. In concert with a beam propagation model that takes into account multiple scattering, this combination of illumination- and detection-side coding allows us to more stably reconstruct 3D phase for larger-scale biological samples. [1] E. Wolf, “Three-dimensional structure determination of semi-transparent objects from holographic data,” Opt. Commun. 1, 153–156 (1969). [2] L. Tian and L. Waller, “3D intensity and phase imaging from light field measurements in an LED array microscope,” Optica, 2, 104-111 (2015).

Paper Details

Date Published: 13 March 2019
Proc. SPIE 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, 108830V (13 March 2019); doi: 10.1117/12.2516567
Show Author Affiliations
Regina Eckert, Univ. of California, Berkeley (United States)
Michael Chen, Univ. of California, Berkeley (United States)
Li-Hao Yeh, Univ. of California, Berkeley (United States)
Laura Waller, Univ. of California, Berkeley (United States)

Published in SPIE Proceedings Vol. 10883:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI
Thomas G. Brown; Tony Wilson, Editor(s)

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