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Proceedings Paper

Multispectral autoflourescence detection of skin neoplasia using steady-state techniques
Author(s): Ekaterina Borisova; Alexander Gisbrecht; Tsanislava Genova-Hristova; Petranka Troyanova; Elmira Pavlova; Nikolay Penkov; Ivan Bratchenko; Valery Zakharov; Ilze Lihachova; Ilona Kuzmina; Janis Spigulis
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Paper Abstract

In the current study were used excitation-emission matrices (EEMs) and synchronous fluorescence spectroscopy (SFS) steady-state techniques in a broad spectral regions (excitation at 220-500 nm and emission at 280-850 nm) to achieve the whole set of endogenous fluorophores, existed in normal and neoplastic cutaneous tissues. Several types of benign, dysplastic and malignant types of skin lesions were investigated ex vivo using both EEM and SFS modalities, namely the basal cell papilloma and carcinoma, pigmented nevi, dysplastic nevi, squamous cell carcinoma and malignant melanoma. Histological analysis was used as a “gold standard” for evaluation of clinical diagnosis of the lesions investigated. Comparison with the normal skin tissue spectral data was made, based on the signals detected from the safety margins areas of the surgically excised tumours. EEM and SFS data reveal statistically significant differences between variety of benign, dysplastic and malignant lesions, which could be used as fingerprints, applicable for differentiation algorithms. In a few of malignancies endogenous porphyrins signals were even observed, but in general the fluorescence signals were addressed to the coenzymes, such as NADH, flavins; structural proteins, such as collagen, elastin and their cross-links, as well as keratin in the case of basal cell lesions. Pigments, such as hemoglobin and melanin distorted the signal due to intrinsic fluorophores signal reabsorption, what has to be taken into account when the algorithms for discrimination of the pathology types are developed.

Paper Details

Date Published: 29 January 2019
PDF: 6 pages
Proc. SPIE 11047, 20th International Conference and School on Quantum Electronics: Laser Physics and Applications, 1104704 (29 January 2019); doi: 10.1117/12.2516337
Show Author Affiliations
Ekaterina Borisova, Institute of Electronics (Bulgaria)
Saratov State Univ. (Russian Federation)
Alexander Gisbrecht, Institute of Electronics (Bulgaria)
Tsanislava Genova-Hristova, Institute of Electronics (Bulgaria)
Petranka Troyanova, Univ. Hospital "Tsaritsa Yoanna-ISUL" (Bulgaria)
Elmira Pavlova, Univ. Hospital "Tsaritsa Yoanna-ISUL" (Bulgaria)
Nikolay Penkov, Univ. Hospital "Tsaritsa Yoanna-ISUL" (Bulgaria)
Ivan Bratchenko, Samara National Research Univ. (Russian Federation)
Valery Zakharov, Samara National Research Univ. (Russian Federation)
Ilze Lihachova, Univ. of Latvia (Latvia)
Ilona Kuzmina, Univ. of Latvia (Latvia)
Janis Spigulis, Univ. of Latvia (Latvia)


Published in SPIE Proceedings Vol. 11047:
20th International Conference and School on Quantum Electronics: Laser Physics and Applications
Tanja N. Dreischuh; Latchezar A. Avramov, Editor(s)

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