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Proceedings Paper

Volumetric stimulated Raman imaging with a high-speed deformable mirror (Conference Presentation)

Paper Abstract

High resolution volumetric stimulated Raman scattering (V-SRS) imaging allows a precise measurement of chemical distribution in a three-dimensional (3-D) complex biological system. To compile a stack of multiplane images, current methods such as using piezo objective positioners or tunable lenses either yield low scanning speed, disturbance of specimen, or significant aberrations. Here, we develop a V-SRS microscope with a high-speed MEMS deformable mirror (DM) which has 140 actuators and a frame rate of 20 kHz using hardware-trigger. The DM conjugated with the objective pupil plane enables wavefront shaping at reflectance mode and remote focusing of both pump and Stokes beams on the sample. The depth scan range can reach tens of micrometers by using 40X and 25X objectives. Multiple 3-D cancerous cell images are obtained. We expect the V-SRS to have great potential to enable label-free studies of cell metabolism, brain function, and developmental biology.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108900Q (4 March 2019); doi: 10.1117/12.2513208
Show Author Affiliations
Peng Lin, Boston Univ. (United States)
Hongli Ni, Boston Univ. (United States)
Huate Li, Boston Univ. (United States)
Fengyuan Deng, Boston Univ. (United States)
Nicholas A. Vickers, Boston Univ. (United States)
Yunyin Tang, Boston Univ. (United States)
Thomas G. Bifano, Boston Univ. (United States)
Ji-Xin Cheng, Boston Univ. (United States)

Published in SPIE Proceedings Vol. 10890:
Label-free Biomedical Imaging and Sensing (LBIS) 2019
Natan T. Shaked; Oliver Hayden, Editor(s)

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