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Optimized 3DISCO for imaging of heme-rich tissues by decolorization
Author(s): Yilin Ma; Tingting Yu; Jianyi Xu; Peng Wan; Yisong Qi; Wei Feng; Dan Zhu
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Paper Abstract

Tissue optical clearing technique plays an important role in three-dimensional (3D) visualization of intact organs or rodent bodies. As a typical organic-solvent based clearing method, 3DISCO renders the turbid tissues transparent by the strategy of minimizing light scattering, and possesses the advantages of high transparency and substantial size reduction, which could facilitate imaging of large-volume tissues. However, for heme-rich tissues, such as spleen, liver, embryo and clinical biopsy samples, tissue transparency and image quality by 3DISCO are limited due to the strong absorption ability of hemes. To address this problem, we proposed an optimized clearing method by introducing decolorization to modify the original 3DISCO protocol. The results showed that the optimized protocol could enhance the transparency of the heme-rich tissues and facilitate high-resolution imaging inside these tissues combining with optical microscopies.

Paper Details

Date Published: 1 March 2019
PDF: 5 pages
Proc. SPIE 10877, Dynamics and Fluctuations in Biomedical Photonics XVI, 108770P (1 March 2019); doi: 10.1117/12.2512690
Show Author Affiliations
Yilin Ma, Huazhong Univ. of Science and Technology (China)
Tingting Yu, Huazhong Univ. of Science and Technology (China)
Jianyi Xu, Huazhong Univ. of Science and Technology (China)
Peng Wan, Huazhong Univ. of Science and Technology (China)
Yisong Qi, Huazhong Univ. of Science and Technology (China)
Wei Feng, Huazhong Univ. of Science and Technology (China)
Dan Zhu, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 10877:
Dynamics and Fluctuations in Biomedical Photonics XVI
Valery V. Tuchin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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