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Proceedings Paper

3D histopathology speckle phase contrast imaging: from synchrotron to conventional sources
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Paper Abstract

Standard histopathological examination is the gold standard for many disease diagnoses although the technique is limited with no full 3D volume analysis possible. Three dimensional X-ray Phase-Contrast Imaging(PCI) methods have been under constant and fast developments in the recent decades due to their superior performance for imaging low density objects and their ability to provide complementary information compared to attenuation based imaging. Despite the progresses, X-ray Phase Contrast Tomography still encounters remaining challenges to overcome on its way to become a routine non-invasive technique allowing the 3D assessment of tissue architecture in laboratory set-ups. Speckle Based Imaging (SBI) forms a new class of X-ray PCI techniques, sensitive to the first derivative of the phase. The set-up involved and the simplicity of implementation provide many advantages to SBI such as having no field of view and no resolution limitation in addition to have low requirements on the beam coherences. These advantages make SBI a good candidate for the transfer on conventional sources. In this work, we present preliminary results obtained on a conventional μCT and their comparison with data acquired at the European Synchrotron. We used a new phase retrieval algorithm based on optical energy conservation. We applied the method on both phantoms and biological samples in order to evaluate its quantitativeness for a transfer. A comparison to previously available speckle tracking algorithms is also performed. We demonstrate that the combination of the phase retrieval method with a standard μCT can achieve high resolution and high contrast within a few minutes, with a comparable image quality to the results obtained using synchrotron light.

Paper Details

Date Published: 1 March 2019
PDF: 7 pages
Proc. SPIE 10948, Medical Imaging 2019: Physics of Medical Imaging, 109481S (1 March 2019); doi: 10.1117/12.2511944
Show Author Affiliations
Helene Labriet, Novitom (France)
Sebastien Berujon, ESRF - The European Synchrotron (France)
Ludovic Broche, Univ. Grenoble Alpes (France)
Barbara Fayard, Novitom (France)
Sylvain Bohic, Univ. Grenoble Alpes (France)
Olivier Stephanov, CHU Grenoble (France)
David M. Paganin, Monash Univ. (Australia)
Pierre Lhuissier, Science et Ingénierie des Matériaux et Procédés (France)
Luc Salvo, Science et Ingénierie des Matériaux et Procédés (France)
Sam Bayat, CHU Grenoble (France)
Emmanuel Brun, Univ. Grenoble Alpes (France)

Published in SPIE Proceedings Vol. 10948:
Medical Imaging 2019: Physics of Medical Imaging
Taly Gilat Schmidt; Guang-Hong Chen; Hilde Bosmans, Editor(s)

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