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Proceedings Paper

Human brain glioma grading using label free laser-induced fluorescence spectroscopy
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Paper Abstract

Laser-induced fluorescence (LIF) technique was used to generate spectral signatures of endogenous fluorophores relevant to the tissue molecular composition changes in human brain glioma tumors. The goal is to study the changes of fluorescence emission spectra from endogenous fluorophores in human brain glioma of different grades, and to find new biomarkers for prognostic optical molecular pathological diagnosis. Two hundred and thirty-seven (237) native fluorescence spectra from 61 subjects were measured using LabRAM HR Evolution micro photoluminescence (PL) system for four grades of glioma tumors in ex-vivo. The differences of four grades of glioma tumors were identified by the characteristic fluorophores fingerprints under the excitation laser wavelength at UV 325nm. To our best knowledge, this is the first report for human brain study using this technique. The fluorescence peaks of biomarkers with major contribution were found, including tryptophan, collagen, elastin, reduced nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD) and phospholipids that play important roles in the cellular energy metabolism and glycolysis pathway. The ratios of peak intensities and the peak positions in fluorescence spectra of may be used to diagnose human brain diseases or to guide biopsy during surgical resection.

Paper Details

Date Published: 4 March 2019
PDF: 7 pages
Proc. SPIE 10873, Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 108731A (4 March 2019); doi: 10.1117/12.2511687
Show Author Affiliations
Yan Zhou, The General Hospital of the Air Force, PLA (China)
Cheng-hui Liu, The City Univ. of New York (United States)
Ke Zhu, Institute of Physics (China)
Binlin Wu, Southern Connecticut State Univ. (United States)
Xinguang Yu, The General Hospital of the Air Force, PLA (China)
Gangge Cheng, The General Hospital of the Air Force, PLA (China)
Mingyue Zhao, The General Hospital of the Air Force, PLA (China)
Rui Zong, The General Hospital of the Air Force, PLA (China)
Chunyuan Zhang, The City College of New York (United States)
Hong Chen, China Academy of Space Technology (China)
Shenglin Li, China Academy of Space Technology (China)
Qijun Liang, China Academy of Space Technology (China)
Mingqian Zhang, China Academy of Space Technology (China)
Robert R. Alfano, The City College of New York (United States)


Published in SPIE Proceedings Vol. 10873:
Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos; Angela B. Seddon, Editor(s)

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