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Proceedings Paper

Multi-site optical recording of neuronal activity with complex light patterns
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Paper Abstract

We use complex light patterns to simultaneously record the neuronal activity along the dendrites of a single neuron. We use holographic projection to produce multiple foci directed onto different dendritic regions of the neuron. Each focus excites neuronal activity reporters via either two-photon (2P) or single-photon (1P) excitation. The fluorescence emanating from all foci are simultaneously recorded using an electron-multiplying charge-coupled device (EMCCD) camera thereby enabling simultaneous multi-channel recording of the neuronal activity from multiple sites at high frame rates (up to 400Hz). We report recording of neuronal activity from two types of reporters: (1) Ca2+ indicator, Cal-520; and (2) voltage indicator, JPW-1114. We optically recorded the activity evoked by the neuron following injection of current onto the soma. Holographic multi-site Ca2+ imaging resulted in high signal-to-noise ratio but with poor temporal resolution. On the other hand, multi-site voltage imaging produced noisy and low SNR signals but with high temporal resolution that is able to resolve action potentials.

Paper Details

Date Published: 1 March 2019
PDF: 6 pages
Proc. SPIE 10935, Complex Light and Optical Forces XIII, 109351F (1 March 2019); doi: 10.1117/12.2511683
Show Author Affiliations
Michael Lawrence Castañares, The Australian National Univ. (Australia)
Vincent R. Daria, The Australian National Univ. (Australia)

Published in SPIE Proceedings Vol. 10935:
Complex Light and Optical Forces XIII
Jesper Glückstad; David L. Andrews; Enrique J. Galvez, Editor(s)

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