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Proceedings Paper

Identification of body fluids using metal enhanced fluorescence substrate with glancing angle deposited Ag nanorods (Conference Presentation)
Author(s): Naseem Abbas; Xun Lu; Mohsin ali Badshah; Won il Heo; Sung Jun Seo; Seok-Min Kim

Paper Abstract

The identification of body fluids including blood, saliva, urine, sweat, semen, and vaginal fluid can be a vital evidence that can be used to identify a suspect and reconstructing the criminal case. Since the amount of evidence in the crime site is limited, a multiplex identification system for body fluid using a small amount of sample is prepared. In this research, we proposed a multiplex detection platform for semen and vaginal fluid, which were important for sexual crime using an Ag vertical nanorod metal enhanced fluorescence (Ag-MEF) substrate. The Ag-MEF substrate with a length of 500 nm was fabricated by glancing angle deposition and the Amin functionalization was conducted to improve the binding ability. The effect of incubation time was analyzed and an incubation time of 60 min was selected where the fluorescence signal was saturated. To examine the performance of the developed identification chip, an identification of the semen and virginal fluid was carried out. The developed sensor can selectively identify the semen and virginal fluid without any cross reaction. In addition, the limit of detection for semen was 10 times lower than that of the commercially available RSID-Semen kit.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 108950F (4 March 2019); doi: 10.1117/12.2511045
Show Author Affiliations
Naseem Abbas, Chung-Ang Univ. (Korea, Republic of)
Xun Lu, Chung-Ang Univ. (Korea, Republic of)
Mohsin ali Badshah, Chung-Ang Univ. (Korea, Republic of)
Won il Heo, Chung-Ang Univ. (Korea, Republic of)
Sung Jun Seo, Chung-Ang Univ. (Korea, Republic of)
Seok-Min Kim, Chung-Ang Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 10895:
Frontiers in Biological Detection: From Nanosensors to Systems XI
Amos Danielli; Benjamin L. Miller; Sharon M. Weiss, Editor(s)

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