The fluorescents detector sub-system is one of most important sub-systems of fluorescence-activated cell sorting (FACS) flow cytometry. The Photomultiplier Tube (PMT) detector sub-system are used in the fluorescent detection of Becton Dickinson (BD) AccuriTM C6 Plus. For using cost effective solid-state detectors (SSD) sub-system, we have validated SSD sub-system as the replacement sub-system of PMT. BD Bioscience and Pavilion Integration Corporation (PIC) use BD Instruments (Accuri C6 Plus) as test tools to test the SSD of PIC vs. PMT. We further ran the Swap test and Repeatability as well as ran test at the operational temperature range of cytometer. We also ran the Linearity test. All results were passed. Based on these results, BD Bioscience teams will run the biological sample testing to cover the test of CFSE- Proliferation Assay, Cell Cycle Analysis, and Apoptosis.

Date Published: 27 February 2019
PDF: 10 pages
Proc. SPIE 10870, Design and Quality for Biomedical Technologies XII, 108700A (27 February 2019); doi: 10.1117/12.2510807

Published in SPIE Proceedings Vol. 10870:
Design and Quality for Biomedical Technologies XII
Rongguang Liang; Jeeseong Hwang, Editor(s)