Share Email Print
cover

Proceedings Paper

Development of pulsed blue light technologies for bacterial biofilm disruption
Author(s): Violet V. Bumah; Daniela Masson-Meyers; Dawn Castel; Chris Castel; Chukuka S. Enwemeka
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

In recent studies, we showed that pulsed blue light is more potent in suppressing bacterial growth than continuous wave blue light. The potency of pulsed blue light makes it a viable antimicrobial for suppressing bacteria growth in biofilms, where the protective cover of the biofilm makes it is tougher to suppress bacteria. Consequently, we studied the efficacy of pulsed 450 nm light in suppressing the growth of MRSA and P. acnes biofilms. The results showed 100% bacterial suppression in planktonic cultures of MRSA irradiated with 7.6 J/cm2 three times a day, using 3 mW/cm2 irradiance, and in P. acnes planktonic cultures irradiated with 5 J/cm2 thrice daily for 3 days, using 2 mW/cm2 irradiance. However, a similar 100% suppression was not attained in MRSA or P. acnes biofilms irradiated thrice daily for 3 days at various fluences; but LIVE/DEAD assay showed a degree of bacterial suppression, with more live cells in controls than irradiated biofilms, and more dead cells in irradiated than control biofilms. In addition, while control biofilms had intact biofilm networks, irradiated biofilms had disrupted biofilm. The higher the dose, the more bacterial suppression and biofilm disruption. These findings confirm our previous reports that 100% bacterial suppression is attainable with pulsed blue light, and suggests further modification of the treatment protocol in order to achieve 100% bacterial suppression in biofilms.

Paper Details

Date Published: 7 March 2019
PDF: 8 pages
Proc. SPIE 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 108630U (7 March 2019); doi: 10.1117/12.2510699
Show Author Affiliations
Violet V. Bumah, San Diego State Univ. (United States)
Daniela Masson-Meyers, Marquette Univ. (United States)
Dawn Castel, CareWear Corp. (United States)
Chris Castel, CareWear Corp. (United States)
Chukuka S. Enwemeka, San Diego State Univ. (United States)


Published in SPIE Proceedings Vol. 10863:
Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II
Tianhong Dai; Jürgen Popp; Mei X. Wu M.D., Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray