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Proceedings Paper

Projection multiplexing for enhanced acquisition speed in holographic tomography
Author(s): Arkadiusz Kuś; Maria Baczewska; Michał Ziemczonok; Małgorzata Kujawińska
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Paper Abstract

In holographic tomography (HT), the 3D refractive index distribution within weakly-scattering, phase-only biological object is retrieved. This key property of the technique is one of its most significant strengths compared to labelling-based methods of cell analysis such as fluorescence microscopy. As a consequence, however, it is required to acquire a set of holograms at several viewing directions, which hinders the measurement speed. In this paper we explore the prospect of multiplexing projections in order to decrease the number of scanning positions required for the full measurements. The presented analysis is based on experimental data acquired in a limited-angle holographic tomography system and emulates the performance of a spatial-light-modulator-based system in which multiple projections may be acquired simultaneously by generating a distribution of multiple point sources in the Fourier plane of the condenser lens. For this reason, the increase in acquisition speed strictly depends on the number of multiplexed holograms and results in decreased reconstruction quality. The performance of the system is demonstrated and analyzed with biological objects - human keratinocyte cells.

Paper Details

Date Published: 1 March 2019
PDF: 6 pages
Proc. SPIE 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, 1088318 (1 March 2019); doi: 10.1117/12.2510628
Show Author Affiliations
Arkadiusz Kuś, Warsaw Univ. of Technology (Poland)
Maria Baczewska, Warsaw Univ. of Technology (Poland)
Michał Ziemczonok, Warsaw Univ. of Technology (Poland)
Małgorzata Kujawińska, Warsaw Univ. of Technology (Poland)

Published in SPIE Proceedings Vol. 10883:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI
Thomas G. Brown; Tony Wilson, Editor(s)

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