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Proceedings Paper

A gold nanoparticle bio-optical transponder to dynamically monitor intracellular events (Conference Presentation)
Author(s): Geoffrey F. Strouse

Paper Abstract

The coupling of a gold nanoparticle to peptides and/or nucleic acids can allow imaging of intracellular events when optically labelled by molecular dyes. The use of a gold bio-optical transponder (BOT) capable of simultaneously reporting the timing of intracellular cargo delivery, cargo release from a gold nanoparticle, and subsequent cellular processing provides direct insight into the endo-lysosomal processing of nano-based delivery probes. The paper will explore use of BOT sensors to monitor peptide dependent uptake of AuNPs into native vs drug resistant melanoma cells; monitor the effect off surface coupling of gene knock-in, and explore the ability to evaluate the evolving intracellular pH (pHi) during endosomal maturation. The BOT is designed around use of multicolor surface energy transfer (SET) to simultaneously track processes and provide an internal reference for ratiometric analysis of events. The use of a BOT based sensor to explore intracellular processing may enable greater insight into our understanding of the fundamental processes of biology.

Paper Details

Date Published: 13 March 2019
Proc. SPIE 10892, Colloidal Nanoparticles for Biomedical Applications XIV, 108920V (13 March 2019); doi: 10.1117/12.2510456
Show Author Affiliations
Geoffrey F. Strouse, Florida State Univ. (United States)

Published in SPIE Proceedings Vol. 10892:
Colloidal Nanoparticles for Biomedical Applications XIV
Marek Osiński; Wolfgang J. Parak, Editor(s)

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