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Proceedings Paper

Identification of MRSA infection in blood using photoacoustic flow cytometry
Author(s): Robert H. Edgar; Cierra Noel; Austin Minard; Rachel Fernandez; Matthew Fitzpatrick; Andrea Sajewski; Justin Cook; John D. Hempel; John A. Kellum; John A. Viator
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Paper Abstract

Multi-drug-resistant bacteria, particularly Methicillin resistant, have become an ever-increasing problem. Currently, broad spectrum antibiotics are prescribed until bacteria can be cultured, a process that can take 3-4 days and is unable to deliver quantitative information about relative number of bacteria present. In order to rapidly detect, differentiate and quantify bacteria in blood samples we designed a detection system using labeled bacteriophage in conjunction with photoacoustic flow cytometry. Photoacoustic Flow cytometry is the generation of ultrasound waves created by the absorption of laser light in objects under flow. Bacteria can be tagged with dyed phage and processed through the photoacoustic flow cytometer where they are detected by the acoustic response. Here we demonstrate that E.coli can be detected and discriminated from Salmonella using this method.

Paper Details

Date Published: 27 February 2019
PDF: 9 pages
Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 1087860 (27 February 2019); doi: 10.1117/12.2510210
Show Author Affiliations
Robert H. Edgar, Univ. of Pittsburgh (United States)
Cierra Noel, Duquesne Univ. (United States)
Austin Minard, Duquesne Univ. (United States)
Rachel Fernandez, Duquesne Univ. (United States)
Matthew Fitzpatrick, Duquesne Univ. (United States)
Andrea Sajewski, Duquesne Univ. (United States)
Justin Cook, Duquesne Univ. (United States)
John D. Hempel, Duquesne Univ. (United States)
John A. Kellum, Univ. of Pittsburgh (United States)
John A. Viator, Univ. of Pittsburgh (United States)
Duquesne Univ. (United States)

Published in SPIE Proceedings Vol. 10878:
Photons Plus Ultrasound: Imaging and Sensing 2019
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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