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Proceedings Paper

Multimodal microscopy toolkit for visualizing multicomponent topical drug formulations in humans
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Paper Abstract

Our group has recently developed a method for characterizing distribution of a topical drug within skin using two-photon fluorescence lifetime imaging (FLIM) and phasor analysis. Here, we expand on this work by describing a multimodal approach for rapidly visualizing multiple components in tissue using FLIM and coherent Raman imaging (CRI). By employing a non-Euclidian FLIM phasor analysis for a three-component system informed with the vibrational signature of one of the components retrieved with CRI, we were able to semi-quantitatively describe the spatial distribution of drugs in tissue with molecular specificity and cellular resolution.

Paper Details

Date Published: 5 March 2019
PDF: 5 pages
Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108822J (5 March 2019); doi: 10.1117/12.2510157
Show Author Affiliations
Alexander Fast, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
Sinyoung Jeong, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
Maiko Hermsmeier, BioPharmX, Inc. (United States)
Akira Yamamoto, BioPharmX, Inc. (United States)
Xin Chen, BioPharmX, Inc. (United States)
Kin F. Chan, BioPharmX, Inc. (United States)
Conor L. Evans, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)


Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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