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Proceedings Paper

Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics
Author(s): Priyank Shah; Brad Littleton; Thomas Kavanagh; Yu Nie; Stephen Sturzenbaum; David Richards
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Paper Abstract

Broadband coherent anti-Stokes Raman scattering (B-CARS) in a hybrid 2-color/3-color excitation regime has been shown to be a photon-efficient method of generating CARS across the entire vibrational region of interest (fingerprint and C-H stretch). We extend here our spectral interferometric polarized CARS (SIP-CARS) approach to a hybrid 2-color/3-color excitation regime, demonstrate that the power/polarization dependence for both 2- color and 3-color excitation agrees with theory, explore the application of SIP-CARS for the special case of anisotropic, birefringent materials (using collagen) and present in vivo lipid imaging of different mutations of the nematode Caenorhabditis elegans with modified lipid distributions. The extension of SIP-CARS to a hybrid excitation scheme makes possible a fast, all-optical and quantitative method for Raman imaging.

Paper Details

Date Published: 22 February 2019
PDF: 8 pages
Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108822C (22 February 2019); doi: 10.1117/12.2510052
Show Author Affiliations
Priyank Shah, King's College London (United Kingdom)
Brad Littleton, King's College London (United Kingdom)
Thomas Kavanagh, King's College London (United Kingdom)
Yu Nie, King's College London (United Kingdom)
Stephen Sturzenbaum, King's College London (United Kingdom)
David Richards, King's College London (United Kingdom)

Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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