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Proceedings Paper

All-optical readout and stimulation of cortical activity during optogenetically-triggered motor task in awake mice (Conference Presentation)
Author(s): Francesco Resta; Elena Montagni; Emilia Conti; Giuseppe de Vito; Anna Letizia Allegra Mascaro; Francesco Saverio Pavone

Paper Abstract

In this study, we developed a wide-field all-optical system based on a red-shifted GECI (RCaMP1a) combined with channelrhodopsin II (ChR2) for simultaneous stimulation and readout of neuronal activity. Our results show that RCaMP1a transfection in primary motor cortex extends all over the cortical motor areas. The RCaMP1a and ChR2 reporter expression largely overlap, thus allowing the stimulation and readout from the same functional areas. Furthermore, we characterized the functional response by performing single pulse optogenetic stimulation and we observed that evoked calcium signals increase at increasing laser power. In order to study the cortical activation underlying a specific motor behavior, we performed optogenetic-stimulation of the Rostral Forelimb Area (RFA) with a train of lasers pulses. We observed that during 1s of 16 Hz train stimulus the animals suddenly start grasping with the contralateral forelimb. Cortical dynamics recorded during the optogenetically-triggered motor task show correlated activity between the RFA and the nearby motor areas. The all-optical system optimization and the possibility to link the neuronal population activity with the animal behavior would be a key point in understanding the network activity underlying a specific behavior.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10865, Neural Imaging and Sensing 2019, 108650Y (4 March 2019); doi: 10.1117/12.2508322
Show Author Affiliations
Francesco Resta, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Elena Montagni, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Emilia Conti, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Giuseppe de Vito, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Anna Letizia Allegra Mascaro, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Instituto di Neuroscienze, Consiglio Nazionale delle Ricerche (Italy)
Francesco Saverio Pavone, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy)

Published in SPIE Proceedings Vol. 10865:
Neural Imaging and Sensing 2019
Qingming Luo; Jun Ding; Ling Fu, Editor(s)

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