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Proceedings Paper

Time-lens based multi-color background-free coherent anti-Stokes Raman scattering microscopy
Author(s): Yifan Qin; Bo Li; Fei Xia; Yuanqin Xia; Chris Xu
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Paper Abstract

We demonstrate a multi-color background-free coherent anti-Stokes Raman scattering (CARS) imaging system, using a low-cost, all-fiber, energetic, multi-wavelength time-lens source. The time-lens source generates picosecond pulse trains at three different wavelengths. The first is 1064.3 nm, the second is tunable between 1052 nm and 1055 nm, and the third is tunable between 1040 nm and 1050 nm. When the time-lens source is synchronized with a mode-locked Ti:Sa laser, two of the three wavelengths are used to detect different Raman frequencies for two-color on-resonance imaging, whereas the third wavelength is used to obtain the off-resonance image for nonresonant background subtraction. Mixed poly(methyl methacrylate) (PMMA) and polystyrene (PS) beads are used to demonstrate two-color background-free CARS imaging. Synchronization of the multi-wavelength time-lens source with a microscope enables pixel-to-pixel wavelength-switching. Simultaneous two-color CARS imaging of CH2 and CH3 stretching vibration modes with realtime background subtraction is demonstrated in ex vivo mouse tissue.

Paper Details

Date Published: 4 March 2019
PDF: 6 pages
Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108901U (4 March 2019); doi: 10.1117/12.2507990
Show Author Affiliations
Yifan Qin, Harbin Institute of Technology (China)
Cornell Univ. (United States)
Bo Li, Cornell Univ. (United States)
Fei Xia, Cornell Univ. (United States)
Yuanqin Xia, Harbin Institute of Technology (China)
Chris Xu, Cornell Univ. (United States)

Published in SPIE Proceedings Vol. 10890:
Label-free Biomedical Imaging and Sensing (LBIS) 2019
Natan T. Shaked; Oliver Hayden, Editor(s)

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