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Proceedings Paper

Fast label-free photoacoustic-histology (Conference Presentation)

Paper Abstract

A Frozen section examination is the conventional intraoperative histology method widely used in cancer surgery for tumor margin assessment. However, this method is necessary to perform complicated process including sectioning and staining, which require approximately 15 minutes. Particularly with the ultraviolet (UV) laser (266 nm), photoacoustic microscopy (PAM) showed the capability to visualize cell nuclei without the time consuming procedures by utilizing superior optical contrast of DNA/RNA at this wavelength, which can be a potential alternative of the frozen section. However, previously developed UV-PAM is limited to be applied in intraoperative scenarios because it has suffered from slow imaging speed because of 2D mechanical scanning with linear stepper motors. To overcome this limitation, we developed a fast UV-PAM system based on a 2-axis waterproof microelectromechanical systems (MEMS) scanner with the specially fabricated optical components for UV light. This MEMS scanner enables to scan 3 × 3 mm2 range and acquire 400 × 400 pixels image within 20 seconds. The measured spatial and axial resolutions of the developed system are 2.2 and 39 μm, respectively. Finally, we acquired the histology-like PA image of the mouse kidney with characteristic tubular structures of kidney epithelial cells. In the mouse brain, distinct microstructures such as hippocampus and dentate gyrus were differentiated with the validation of frozen section sample.

Paper Details

Date Published: 4 March 2019
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Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 108781V (4 March 2019); doi: 10.1117/12.2507837
Show Author Affiliations
Jin Woo Baik, Pohang Univ. of Science and Technology (Korea, Republic of)
Jin Young Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
Hyojin Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
Hyosang Ahn, Gachon Univ. of Medicine and Science (Korea, Republic of)
Kyunghee Byun, Gachon Univ. of Medicine and Science (Korea, Republic of)
Hae Young Choi, Daegu-Gyeongbuk Medical Innovation Foundation (Korea, Republic of)
Seon Young Ryu, Pohang Univ. of Science and Technology (Korea, Republic of)
Chulhong Kim, Pohang Univ. of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 10878:
Photons Plus Ultrasound: Imaging and Sensing 2019
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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