Share Email Print

Proceedings Paper

Combined confocal and photoacoustic microscopy based on probe beam deflection technique
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

A device for acquiring co-registered reflectance confocal microscopy (RCM) and optical-resolution photoacoustic microscopy (OR-PAM) images is presented. It uses a pulsed laser for photoacoustic excitation and a continuous laser for the confocal mode, co-aligned, focused and scanned over planes within the object. The photoacoustic part uses a probe beam deflection method, where a continuous laser beam is bent in the refractive index gradient generated by the pressure wave. First images on a carbon microfiber phantom demonstrate the capability of generating co-registered images, where the RCM mode displays back-scattering contrast, while the OR-PAM mode creates images with optical absorption contrast. In the temporal signals of both methods, typical signatures of transient cavitation were visible in the carbon fiber experiment. A lateral resolution of the setup of about 1.6 μm and a noise equivalent pressure amplitude of 90 Pa for the probe beam deflection method were measured.

Paper Details

Date Published: 27 February 2019
PDF: 6 pages
Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 108784O (27 February 2019); doi: 10.1117/12.2507502
Show Author Affiliations
Robert Nuster, Karl-Franzens-Univ. Graz (Austria)
Rainer Hofmann-Wellenhof, Medizinischen Univ. Graz (Austria)
Guenther Paltauf, Karl-Franzens-Univ. Graz (Austria)

Published in SPIE Proceedings Vol. 10878:
Photons Plus Ultrasound: Imaging and Sensing 2019
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

© SPIE. Terms of Use
Back to Top