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Proceedings Paper

Effect of oxidative stress stimulation on intracellular PTEN trafficking in ovarian cancer by fluorescence correlation spectroscopy
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Paper Abstract

The phosphatase and tensin homolog on chromosome 10 (PTEN) is one of important tumor suppressor proteins in ovarian cancer via negatively regulating the phosphatidylinositol 3-kinase–AKT signaling pathway and controlling genomic stability. Recent studies showed the physiological function of PTEN was closely related with its subcellular compartments. But only a few technologies could quantitatively measure the concentration of PTEN at different subcellular compartments in living cells. In this study, we used fluorescence correlation spectroscopy to measure the concentrations and dynamics of EGFP-PTEN in ovarian cancer cells HO-8910. Our results showed the increasing concentration of PTEN in the cytoplasm had an opposite trends with the nucleus after the oxidative stress stimulation which was induced by H2O2. Furthermore, the altered diffusion of PTEN at different subcellular compartments also illustrated the PTEN was trafficked from the cytoplasm to nucleus.

Paper Details

Date Published: 23 October 2018
PDF: 6 pages
Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108202R (23 October 2018); doi: 10.1117/12.2501147
Show Author Affiliations
Shiqing Dong, Fujian Normal Univ. (China)
Xiaoqiong Tang, Fujian Normal Univ. (China)
Jiao Wang, Fujian Normal Univ. (China)
Jianling Chen, Fujian Normal Univ. (China)
Zhengchao Wang, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 10820:
Optics in Health Care and Biomedical Optics VIII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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