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Proceedings Paper

Antibody-based fiber optic sensors for environmental and process-control applications
Author(s): Steven M. Barnard; David R. Walt
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Paper Abstract

Combining fluoroimmunoassay principles and fiber-optic fluorimetry has extended the available transduction schemes for development of fiber-optic sensors. Three representative designs are discussed focusing on a sensor configuration utilizing a controlled-release polymer to sustain a release of indicating reagents over long periods. This novel approach has been adapted for pesticide monitoring. A model system has been designed that releases the reagents of a homogeneous fluoroimmunoassay from an ethylene vinyl acetate polymer for the detection of atrazine. The sensor utilizes a competition reaction between fluorescent-labeled and unlabeled atrazine for the available binding sites on labeled anti-atrazine antibodies.

Paper Details

Date Published: 1 March 1991
PDF: 12 pages
Proc. SPIE 1368, Chemical, Biochemical, and Environmental Fiber Sensors II, (1 March 1991); doi: 10.1117/12.24800
Show Author Affiliations
Steven M. Barnard, Tufts Univ. (United States)
David R. Walt, Tufts Univ. (United States)

Published in SPIE Proceedings Vol. 1368:
Chemical, Biochemical, and Environmental Fiber Sensors II
Robert A. Lieberman; Marek T. Wlodarczyk, Editor(s)

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