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Proceedings Paper

Three-dimensional reconstruction and lateral views in optical microscopy
Author(s): Tullio Tommasi; Bruno Bianco; Vittorio Murino; Alessandra Oneto; Alberto Diaspro
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Paper Abstract

The three-dimensional properties of an optical microscope are analyzed and a defocusing technique is proposed to recover the spatial distribution of the specimens under investigation. Limitations and real capabilities of the 3D reconstruction are pointed out. As a result, lateral views are obtained by means of operations in the spatial frequency domain. In such a way, it is possible to represent side views of an object within the angular aperture range of the microscope. A theory concerning image formation is discussed and simulations of side view reconstructions are reported.

Paper Details

Date Published: 1 September 1990
PDF: 6 pages
Proc. SPIE 1360, Visual Communications and Image Processing '90: Fifth in a Series, (1 September 1990); doi: 10.1117/12.24251
Show Author Affiliations
Tullio Tommasi, Univ. of Genoa (Italy)
Bruno Bianco, Univ. of Genoa (Italy)
Vittorio Murino, Univ. of Genoa (Italy)
Alessandra Oneto, Univ. of Genoa (Italy)
Alberto Diaspro, Univ. of Genoa (Italy)

Published in SPIE Proceedings Vol. 1360:
Visual Communications and Image Processing '90: Fifth in a Series
Murat Kunt, Editor(s)

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