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Proceedings Paper

Fiber optic light-scattering measurement system for evaluation of embryo viability: model experiment
Author(s): Harumi Itoh; Tsunenori Arai; Makoto Kikuchi
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Paper Abstract

We evaluated the particle density detectability and particle size detectivity of our fiber-optic light-scattering measurement system. In order to prevent the multiple pregnancy on current in vitro fertilization-embryo transfer, we have aimed to develop a new quantitative and non- invasive method to select a single viable human embryo. We employed the measurement of mitochondria localization in an embryo, which may have the correlation with development ability. We applied the angular distribution measurement of the light-scattering intensity from the embryo to obtain the information originated from the mitochondria. The latex spheres with a diameter of 1.0 micrometers were used to simulate the scattering intensity of the mitochondria. The measurement probes of our system consisted of two fibers for illumination and sensing. They were arranged at a right angle to a microscope optical axis to measure the angular distribution of the light-scattering intensity. We observed that the light-scattering intensity increased monotonically in the range from 106 to 1010 particles per ml. Since the mitochondria density in a human embryo corresponded to 2.5 X 107 per ml in the measurement chamber, we may measure the mitochondria density in the human embryo. The angular dependence of light-scattering intensity changed with the sphere diameters. This result showed the possibility of the selective measurement of the mitochondria density in the embryo in spite of the presence of the other cell organelle. We think that our light-scattering measurement system might be applicable to the evaluation method for the embryo viability.

Paper Details

Date Published: 7 May 1996
PDF: 5 pages
Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); doi: 10.1117/12.239586
Show Author Affiliations
Harumi Itoh, National Defense Medical College (Japan)
Tsunenori Arai, National Defense Medical College (Japan)
Makoto Kikuchi, National Defense Medical College (Japan)

Published in SPIE Proceedings Vol. 2681:
Laser-Tissue Interaction VII
Steven L. Jacques, Editor(s)

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