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Proceedings Paper

Application of the method of partial least squares to determine chromophore concentrations from fluorescence spectra of turbid samples
Author(s): Anthony J. Durkin; Rebecca R. Richards-Kortum
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Paper Abstract

An non-invasive optical method for obtaining quantitative biochemical information is desirable. While quantitative methods for predicting fluorophore concentrations in dilute solutions are well known, human tissue is highly scattering and absorbing and cannot be accurately treated with these techniques. We have employed the method of partial least squares (PLS) to develop an empirical, linear model of sample fluorescence from a training set with optical properties and known concentrations representative of those to be predicted. This model can be applied to predict chromophore concentrations in the unknown samples.

Paper Details

Date Published: 10 May 1996
PDF: 10 pages
Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); doi: 10.1117/12.239536
Show Author Affiliations
Anthony J. Durkin, Ctr. for Devices and Radiological Health/FDA (United States)
Rebecca R. Richards-Kortum, Univ. of Texas/Austin (United States)

Published in SPIE Proceedings Vol. 2678:
Optical Diagnostics of Living Cells and Biofluids
Daniel L. Farkas; Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Bruce J. Tromberg, Editor(s)

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