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Proceedings Paper

Photonic effects on mononuclear leukocytes
Author(s): Xiaoyan Dai; Richard B. Beard; Michael A. DellaVecchia M.D.; Alexander V. Priezzhev
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Paper Abstract

The effects of pulsed light (2 Hz) with a 55% duty ratio and continuous light on the migration of human mononuclear leukocytes, MNLs (monocytes and lymphocytes) are reported for red light ((lambda) equals 660 nm) and green light ((lambda) equals 565 nm). The comparison of the relative value of the distance to blood cell migration under light to the control cell migration without light stimulus is recorded as cytokinetic index, K.I. K.I. is a measure of the cytokinesis which is the progress of the cell movement in which the migration is enhanced by substances in the cell environment irrespective of a concentration gradient. Red light stimulation produces K.I.'s for PMNs which are 30% grater than for MNLs. Green light stimulation produces K.I.'s for PMNs less than 1.0 indicative of inhibited migration, while for MNLs the K.I.'s are slightly greater than 1.0 indicative of enhanced migration.

Paper Details

Date Published: 10 May 1996
PDF: 8 pages
Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); doi: 10.1117/12.239517
Show Author Affiliations
Xiaoyan Dai, Drexel Univ. (United States)
Richard B. Beard, Drexel Univ. (United States)
Michael A. DellaVecchia M.D., Wills Eye Hospital (United States)
Alexander V. Priezzhev, M.V. Lomonosov Moscow State Univ. (Russia)


Published in SPIE Proceedings Vol. 2678:
Optical Diagnostics of Living Cells and Biofluids
Daniel L. Farkas; Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Bruce J. Tromberg, Editor(s)

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